Hotspot

trnV-UAC-trnM-CAU

LSC · 53464-53669 · 206 bp

Back to Stenotaphrum

Pi 0.0291

MarkerSeek score 69.20

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 206
variable_sites: 6
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	282-287 bp	1.0	75.3
trnV-UAC-trnM-CAU_p2	CGAGTTGCTCTACCGAACTG	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.4	57.3	281-286 bp	1.0	73.3
trnV-UAC-trnM-CAU_p3	CCGAACTGAGCTATAGCCCT	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.7	58.4	270-275 bp	1.0	73.1
trnV-UAC-trnM-CAU_p4	CGAGTTGCTCTACCGAACTG	TTTGAACCAATGACTCCCGC	55.0	50.0	58.4	58.8	297-302 bp	1.0	72.5
trnV-UAC-trnM-CAU_p5	CGAGTTGCTCTACCGAACTG	AGCGAAGTAAGTCCAATGCT	55.0	45.0	58.4	57.2	354-359 bp	1.0	72.1