Hotspot

trnL-UAA-trnF-GAA

LSC · 49532-49809 · 278 bp

Back to Stenotaphrum

Pi 0.0328

MarkerSeek score 65.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 9
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TCAAGTCCCTCTATCCCCAC	TTTTTCTGTGCCAGGAACCA	55.0	45.0	57.8	57.8	378-382 bp	1.0	81.2
trnL-UAA-trnF-GAA_p2	TCAAGTCCCTCTATCCCCAC	TTTGAACTGGTGACACGAGG	55.0	50.0	57.8	58.1	356-360 bp	1.0	81.1
trnL-UAA-trnF-GAA_p3	TCAAGTCCCTCTATCCCCAC	CTGTGCCAGGAACCAGATTT	55.0	50.0	57.8	58.1	373-377 bp	1.0	80.7
trnL-UAA-trnF-GAA_p4	TCAAGTCCCTCTATCCCCAC	GTGCCAGGAACCAGATTTGA	55.0	50.0	57.8	58.1	371-375 bp	1.0	80.7
trnL-UAA-trnF-GAA_p5	TCAAGTCCCTCTATCCCCAC	TTTTCTGTGCCAGGAACCAG	55.0	50.0	57.8	58.3	377-381 bp	1.0	79.7