Hotspot

trnC-GCA-rpoB

LSC · 20150-21333 · 1184 bp

Back to Stenotaphrum

Pi 0.0192
MarkerSeek score 66.60
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1184
variable_sites
22
pi
0.0192
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 TTATTGATTAGGCGGCACCC CCGGGTATTGTGGACATTCC 50.0 55.0 58.0 58.3 1279-1305 bp 1.0 81.1
trnC-GCA-rpoB_p2 TTATTGATTAGGCGGCACCC CGGGTATTGTGGACATTCCC 50.0 55.0 58.0 58.3 1278-1304 bp 1.0 81.1
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1242-1268 bp 1.0 80.9
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1241-1267 bp 1.0 80.9
trnC-GCA-rpoB_p5 TTATTGATTAGGCGGCACCC TCAAGTTCTTCTGCCAAGCC 50.0 50.0 58.0 58.4 1345-1371 bp 1.0 80.4