Hotspot

petN-trnC-GCA

LSC · 19137-20078 · 942 bp

Back to Stenotaphrum

Pi 0.0206

MarkerSeek score 64.70

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 19
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	997-1010 bp	1.0	79.9
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	996-1009 bp	1.0	79.9
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	998-1011 bp	1.0	79.9
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	995-1008 bp	1.0	79.9
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	TGCAAATCCTTTACCCCCAG	60.0	50.0	58.3	57.8	1052-1065 bp	1.0	78.7