Hotspot

psbM-petN

LSC · 18307-19046 · 740 bp

Back to Stenotaphrum

Pi 0.0235

MarkerSeek score 63.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 17
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	847-885 bp	1.0	71.5
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	791-829 bp	1.0	70.7
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	790-828 bp	1.0	70.0
psbM-petN_p4	AAACAGCCAGCCAAAATGAT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	846-884 bp	1.0	69.7
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	845-883 bp	1.0	69.7