Hotspot

trnS-UGA-psbZ

LSC · 11815-12159 · 345 bp

Back to Stenotaphrum

Pi 0.0348

MarkerSeek score 67.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 12
pi: 0.0348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	465-466 bp	1.0	80.3
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	467-468 bp	1.0	79.8
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	485-486 bp	1.0	79.3
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGGAGAAGC	50.0	55.0	57.9	58.3	487-488 bp	1.0	78.9
trnS-UGA-psbZ_p5	GCTATCAACCACTCAGCCAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.7	57.8	470-471 bp	1.0	77.7