Hotspot

ycf10/cemA

LSC · 60229-60918 · 690 bp

Back to Stenopetalum

Pi 0.0157

MarkerSeek score 54.70

Primer available yes

Label ycf10/cemA

Feature Metadata

label_name: ycf10/cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 20
pi: 0.0157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf10/cemA_p1	AGGTCAAGGGGCTATTCCTT	AGTTTGCATGGTCCAATCGT	50.0	45.0	58.0	58.1	1459-1468 bp	1.0	81.9
ycf10/cemA_p2	TTGGCTTACTTCTTGCGTGT	AGTTTGCATGGTCCAATCGT	45.0	45.0	58.0	58.1	1383-1392 bp	1.0	81.7
ycf10/cemA_p3	AGGTCAAGGGGCTATTCCTT	TTCCCTAGCCACAATTCTGC	50.0	50.0	58.0	57.9	1392-1401 bp	1.0	81.7
ycf10/cemA_p4	GTGCGTATTTTTCGTTGGGG	AGTTTGCATGGTCCAATCGT	50.0	45.0	58.0	58.1	1596-1605 bp	1.0	81.5
ycf10/cemA_p5	AGGTCAAGGGGCTATTCCTT	CTCGGGTGAAATACGATGGG	50.0	55.0	58.0	58.1	1839-1840 bp	0.5	59.3