Hotspot

ycf1

SSC · 123595-128949 · 5355 bp

Back to Stenopetalum

Pi 0.013

MarkerSeek score 49.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5355
variable_sites: 132
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACTTCGTAACCAGTTTTTCCA	TCACAAGCATATGTATTTTACAA	40.9	26.1	56.9	52.5	217 bp	1.0	43.7
ycf1_p2	TGACTTCGTAACCAGTTTTTCC	TCACAAGCATATGTATTTTACAA	40.9	26.1	56.9	52.5	218 bp	1.0	43.7
ycf1_p3	GACTTCGTAACCAGTTTTTCCA	TCACAAGCATATGTATTTTACAAA	40.9	25.0	56.9	53.3	217 bp	1.0	43.7
ycf1_p4	TGACTTCGTAACCAGTTTTTCC	TCACAAGCATATGTATTTTACAAA	40.9	25.0	56.9	53.3	218 bp	1.0	43.7
ycf1_p5	ACTTCGTAACCAGTTTTTCCA	TCACAAGCATATGTATTTTACAA	38.1	26.1	55.7	52.5	216 bp	1.0	43.7