Hotspot

trnL-orf77

IRa · 144337-144882 · 546 bp

Back to Stenopetalum

Pi 0.00183

MarkerSeek score 63.60

Primer available yes

Label trnL-orf77

Feature Metadata

label_name: trnL-orf77
feature_type: igs
region: IRa
length_bp: 546
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-orf77_p1	AAATGGTAGACACGCGAGAC	CGCATGTCGGATGAAATTGT	50.0	45.0	58.0	57.2	668-677 bp	1.0	76.2
trnL-orf77_p2	AAATGGTAGACACGCGAGAC	ACGCATGTCGGATGAAATTG	50.0	45.0	58.0	57.2	669-678 bp	1.0	76.1
trnL-orf77_p3	TGAAATGGTAGACACGCGAG	ACGCATGTCGGATGAAATTG	50.0	45.0	58.0	57.2	671-680 bp	1.0	76.1
trnL-orf77_p4	TGAAATGGTAGACACGCGAG	CGCATGTCGGATGAAATTGT	50.0	45.0	58.0	57.2	670-679 bp	1.0	76.1
trnL-orf77_p5	GCCTTGGTGGTGAAATGGTA	CGCATGTCGGATGAAATTGT	50.0	45.0	58.1	57.2	680-689 bp	1.0	75.5