Hotspot

rpl32-trnL

SSC · 113216-114012 · 797 bp

Back to Stenopetalum

Pi 0.0147

MarkerSeek score 64.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 797
variable_sites: 23
pi: 0.0147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TGGACATCGTTGAAAGCTTT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.9	57.9	907-921 bp	1.0	66.5
rpl32-trnL_p2	TTGGACATCGTTGAAAGCTT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.9	57.9	908-922 bp	1.0	66.5
rpl32-trnL_p3	TGGACATCGTTGAAAGCTTT	AGCAGCGTGTCTACCAATTT	40.0	45.0	55.9	57.8	900-914 bp	1.0	66.0
rpl32-trnL_p4	TTGGACATCGTTGAAAGCTT	AGCAGCGTGTCTACCAATTT	40.0	45.0	55.9	57.8	901-915 bp	1.0	66.0
rpl32-trnL_p5	TGGACATCGTTGAAAGCTTT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	55.9	57.8	905-919 bp	1.0	65.9