Hotspot

rps18-rpl20

LSC · 67326-67625 · 300 bp

Back to Stenopetalum

Pi 0.0339

MarkerSeek score 69.30

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 19
pi: 0.0339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GAAAGAAGTGAGTCGACCCC	CGGAAAATACTTGCACAAATAGC	55.0	39.1	57.9	57.1	426-429 bp	1.0	52.0
rps18-rpl20_p2	GAAGTGAGTCGACCCCTAGA	CGGAAAATACTTGCACAAATAGC	55.0	39.1	57.9	57.1	422-425 bp	1.0	51.7
rps18-rpl20_p3	AGAAGTGAGTCGACCCCTAG	CGGAAAATACTTGCACAAATAGC	55.0	39.1	57.9	57.1	423-426 bp	1.0	51.6
rps18-rpl20_p4	AGTGAGTCGACCCCTAGAAC	CGGAAAATACTTGCACAAATAGC	55.0	39.1	58.2	57.1	420-423 bp	1.0	51.4
rps18-rpl20_p5	GTGAGTCGACCCCTAGAACT	CGGAAAATACTTGCACAAATAGC	55.0	39.1	58.2	57.1	419-422 bp	1.0	51.4