Hotspot

rbcL-accD

LSC · 56152-56824 · 673 bp

Back to Stenopetalum

Pi 0.0183

MarkerSeek score 57.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 24
pi: 0.0183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1033-1043 bp	1.0	82.0
rbcL-accD_p2	TCGGTGGAGGAACTTTAGGT	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1122-1132 bp	1.0	81.9
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	ATTCCGAATGTCCTTGGAGC	45.0	50.0	58.0	58.0	1442-1452 bp	1.0	81.6
rbcL-accD_p4	TCATGGTATGCACTTCCGTG	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1409-1419 bp	1.0	81.6
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1609-1619 bp	1.0	81.4