Hotspot

trnD-trnY

LSC · 29841-30266 · 426 bp

Back to Stenopetalum

Pi 0.0184
MarkerSeek score 63.70
Primer available yes
Label trnD-trnY

Feature Metadata

label_name
trnD-trnY
feature_type
igs
region
LSC
length_bp
426
variable_sites
14
pi
0.0184
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-trnY_p1 TGATGTATTGAATCCGCCGG GGGGACGGACTGTAAATTCG 50.0 55.0 58.1 58.4 580-594 bp 1.0 79.2
trnD-trnY_p2 CCTTGCCCCGAAACAAAAAG GGGGACGGACTGTAAATTCG 50.0 55.0 58.1 58.4 606-620 bp 1.0 79.0
trnD-trnY_p3 CTTGCCCCGAAACAAAAAGG GGGGACGGACTGTAAATTCG 50.0 55.0 58.1 58.4 605-619 bp 1.0 79.0
trnD-trnY_p4 TGATGTATTGAATCCGCCGG GGGACGGACTGTAAATTCGT 50.0 50.0 58.1 57.6 579-593 bp 1.0 79.0
trnD-trnY_p5 CTTGCCCCGAAACAAAAAGG GGGACGGACTGTAAATTCGT 50.0 50.0 58.1 57.6 604-618 bp 1.0 78.8