Hotspot

trnR-atpA

LSC · 9529-9802 · 274 bp

Back to Stenopetalum

Pi 0.0217

MarkerSeek score 58.30

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 11
pi: 0.0217
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGGA	47.6	40.9	57.3	57.3	301-357 bp	1.0	51.8
trnR-atpA_p2	GGTTCAAATCCTATTGGACGC	CTGGAACGTTTTCTACTTCAGG	47.6	45.5	57.3	57.0	302-358 bp	1.0	49.2
trnR-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAACGTTTTCTACTTCAGGAGA	47.6	43.5	57.3	58.1	300-356 bp	1.0	48.2
trnR-atpA_p4	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGG	47.6	42.9	57.3	55.8	301-357 bp	1.0	47.9
trnR-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAACGTTTTCTACTTCAGGAG	47.6	45.5	57.3	56.7	300-356 bp	1.0	47.2