Hotspot

trnK-rps16

LSC · 4316-4980 · 665 bp

Back to Stenopetalum

Pi 0.0176

MarkerSeek score 64.20

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 22
pi: 0.0176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	GGGATTTTTACGGAATTAAGTCT	50.0	34.8	58.2	54.7	744 bp	0.25	10.0
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	GGGATTTTTACGGAATTAAGTCT	50.0	34.8	58.2	54.7	743 bp	0.25	10.0
trnK-rps16_p3	AAAGCCGAGTACTCTACCGT	GGGATTTTTACGGAATTAAGTCTT	50.0	33.3	58.2	55.4	744 bp	0.25	10.0
trnK-rps16_p4	AAGCCGAGTACTCTACCGTT	GGGATTTTTACGGAATTAAGTCTT	50.0	33.3	58.2	55.4	743 bp	0.25	10.0
trnK-rps16_p5	TACCGTTGAGTTAGCAACCC	GGGATTTTTACGGAATTAAGTCT	50.0	34.8	57.5	54.7	730 bp	0.25	10.0