Hotspot

ndhH

IRb · 118064-119259 · 1196 bp

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Pi 0.321

MarkerSeek score 71.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: IRb
length_bp: 1196
variable_sites: 59
pi: 0.321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.15
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	29.2	45.0	55.5	58.0	1291 bp	0.5	20.0
ndhH_p2	TGAAGATTGGTTTGAATTTTGTTCT	TAATTTTGTCGCGTCCGCTA	28.0	45.0	56.4	58.0	1349 bp	0.5	20.0
ndhH_p3	TTCATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	28.0	45.0	56.2	58.0	1292 bp	0.5	20.0
ndhH_p4	CATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	30.4	45.0	54.1	58.0	1290 bp	0.5	20.0
ndhH_p5	TCATTTTGATGAACCTCATTTTG	TAATTTTGTCGCGTCCGCTA	30.4	45.0	54.1	58.0	1291 bp	0.5	20.0