Hotspot

ndhA

IRb · 119049-121255 · 2207 bp

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Pi 0.405

MarkerSeek score 68.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: IRb
length_bp: 2207
variable_sites: 254
pi: 0.405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.28
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGGCATTATTGGTGGAGAGG	GCTCGTATTGTTTGTTGGCC	55.0	50.0	58.0	58.0	2705 bp	0.5	59.7
ndhA_p2	TGGGAAGTCCAATGGCAAAA	GCTCGTATTGTTTGTTGGCC	45.0	50.0	57.9	58.0	2584 bp	0.5	59.1
ndhA_p3	TGGGGTTTATCAGGACCGAT	GCTCGTATTGTTTGTTGGCC	50.0	50.0	58.1	58.0	2674 bp	0.5	58.9
ndhA_p4	CTTGGACCTTTTATGGCGGA	GCTCGTATTGTTTGTTGGCC	50.0	50.0	57.9	58.0	2976 bp	0.5	58.8
ndhA_p5	GCTACGAGCTTCTGGAATCC	GCTCGTATTGTTTGTTGGCC	55.0	50.0	58.1	58.0	2654 bp	0.5	58.7