Hotspot

trnH-GUG-rpl2

IRb · 86501-86547 · 47 bp

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Pi 0.0426
MarkerSeek score 77.00
Primer available yes
Label trnH-GUG-rpl2

Feature Metadata

label_name
trnH-GUG-rpl2
feature_type
igs
region
IRb
length_bp
47
variable_sites
2
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl2_p1 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 197 bp 1.0 79.6
trnH-GUG-rpl2_p2 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 183 bp 1.0 79.6
trnH-GUG-rpl2_p3 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 182 bp 1.0 79.6
trnH-GUG-rpl2_p4 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 184 bp 1.0 79.6
trnH-GUG-rpl2_p5 CGGATGTAGCCAAGTGGATC CACAACCCCTTGGGGTTATC 55.0 55.0 58.1 58.2 211 bp 1.0 78.5