Hotspot

rpoA-rps11

LSC · 81004-81071 · 68 bp

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Pi 0.0441

MarkerSeek score 73.70

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 3
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CGGGTGGATCCTGCTATTTC	CATTACGGGCCATTCGTAGA	55.0	50.0	58.1	57.5	204 bp	1.0	76.1
rpoA-rps11_p2	CGGGTGGATCCTGCTATTTC	TACGGGCCATTCGTAGAAGT	55.0	50.0	58.1	58.5	201 bp	1.0	76.1
rpoA-rps11_p3	CCTTTCATGAGTGGGGACAG	CATTACGGGCCATTCGTAGA	55.0	50.0	57.9	57.5	291 bp	1.0	75.7
rpoA-rps11_p4	CCTTTCATGAGTGGGGACAG	TACGGGCCATTCGTAGAAGT	55.0	50.0	57.9	58.5	288 bp	1.0	75.7
rpoA-rps11_p5	CGGGTGGATCCTGCTATTTC	TTACGGGCCATTCGTAGAAG	55.0	50.0	58.1	57.4	202 bp	1.0	75.5