Hotspot

atpB-rbcL

LSC · 56270-57206 · 937 bp

Back to Stenomesson

Pi 0.0202

MarkerSeek score 75.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 18
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	1030-1045 bp	1.0	72.9
atpB-rbcL_p2	CAGGACCGGAAGTAGTAGGA	AGTCTCTGTTTGTGGTGACA	55.0	45.0	57.6	56.6	974-989 bp	1.0	70.8
atpB-rbcL_p3	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	1031-1046 bp	1.0	70.0
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	1030-1045 bp	1.0	69.1
atpB-rbcL_p5	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	1054-1069 bp	1.0	68.5