Hotspot

ycf3-trnS-GGA

LSC · 45791-46431 · 641 bp

Back to Stenomesson

Pi 0.0117

MarkerSeek score 69.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 7
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GGTCGGTAATGCTGGTGAAT	45.0	50.0	56.4	58.0	809-854 bp	1.0	71.7
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GGTCGGTAATGCTGGTGAAT	45.0	50.0	56.4	58.0	810-855 bp	1.0	71.7
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	781-826 bp	1.0	70.8
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	782-827 bp	1.0	70.8
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	730-775 bp	1.0	70.5