Hotspot

trnS-GCU-trnG-GCC

LSC · 8626-9887 · 1262 bp

Back to Stenomesson

Pi 0.0481

MarkerSeek score 79.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1262
variable_sites: 58
pi: 0.0481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1321-1339 bp	1.0	64.8
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1322-1340 bp	1.0	62.7
trnS-GCU-trnG-GCC_p3	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1307-1325 bp	1.0	61.9
trnS-GCU-trnG-GCC_p4	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1302-1320 bp	1.0	61.1
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	TCGAACGAATCACACTTTTACC	50.0	40.9	57.6	57.0	1308-1326 bp	1.0	59.8