Hotspot

ycf1

SSC · 120237-125780 · 5544 bp

Back to Stenogyne

Pi 0.000842

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 7
pi: 0.000842
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACATTTCTTGGTTTTCGAAT	ATTGCATCTCCGAGTTGGAC	28.6	50.0	52.5	58.0	188 bp	1.0	40.9
ycf1_p2	ACATTTCTTGGTTTTCGAATT	ATTGCATCTCCGAGTTGGAC	28.6	50.0	52.5	58.0	187 bp	1.0	40.9
ycf1_p3	AACATTTCTTGGTTTTCGAATT	ATTGCATCTCCGAGTTGGAC	27.3	50.0	53.4	58.0	188 bp	1.0	40.9
ycf1_p4	ACATTTCTTGGTTTTCGAATTT	ATTGCATCTCCGAGTTGGAC	27.3	50.0	53.4	58.0	187 bp	1.0	40.9
ycf1_p5	ACATTTCTTGGTTTTCGAATT	GCATCTCCGAGTTGGACAAA	28.6	50.0	52.5	58.2	184 bp	1.0	40.9