Hotspot

rps16

LSC · 4922-6062 · 1141 bp

Back to Stenogyne

Pi 0.000584

MarkerSeek score 59.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1141
variable_sites: 1
pi: 0.000584
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATATACAAGTCGCTACCCCC	TATGGACAGCTGAACCAGTG	50.0	50.0	56.2	57.2	1251 bp	1.0	59.4
rps16_p2	ATATACAAGTCGCTACCCCC	ATGGACAGCTGAACCAGTGA	50.0	50.0	56.2	58.9	1250 bp	1.0	58.1
rps16_p3	ATATACAAGTCGCTACCCCC	GTCTATGGACAGCTGAACCA	50.0	50.0	56.2	56.9	1254 bp	1.0	57.1
rps16_p4	ATATACAAGTCGCTACCCCC	TGTCTATGGACAGCTGAACC	50.0	50.0	56.2	56.9	1255 bp	1.0	57.1
rps16_p5	ATATACAAGTCGCTACCCCC	CTATGGACAGCTGAACCAGT	50.0	50.0	56.2	56.9	1252 bp	1.0	57.0