Hotspot

ndhA

SSC · 116181-118291 · 2111 bp

Back to Stenogyne

Pi 0.00253

MarkerSeek score 59.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2111
variable_sites: 8
pi: 0.00253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTGACCGGAGTTCAAGAA	GCCGGGTTTTATCAACTTGC	45.0	50.0	57.0	58.0	2347 bp	1.0	72.3
ndhA_p2	TGTTGACCGGAGTTCAAGAA	CGCCGGGTTTTATCAACTTG	45.0	50.0	57.0	57.7	2348 bp	1.0	70.3
ndhA_p3	GTTGACCGGAGTTCAAGAAC	GCCGGGTTTTATCAACTTGC	50.0	50.0	56.7	58.0	2346 bp	1.0	69.6
ndhA_p4	TGTTGACCGGAGTTCAAGAAC	GCCGGGTTTTATCAACTTGC	47.6	50.0	58.4	58.0	2347 bp	1.0	68.7
ndhA_p5	GTTGACCGGAGTTCAAGAAC	CGCCGGGTTTTATCAACTTG	50.0	50.0	56.7	57.7	2347 bp	1.0	67.6