Hotspot

ccsA

SSC · 110901-111872 · 972 bp

Back to Stenogyne

Pi 0.000686

MarkerSeek score 59.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 1
pi: 0.000686
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTGGCGGCATATCATTTCT	CTCGCCAATTCACACAAAGT	45.0	45.0	57.4	56.9	1148 bp	1.0	66.4
ccsA_p2	AGTGGCGGCATATCATTTCT	TATGATTCGACAGGCTCTCG	45.0	50.0	57.4	56.9	1164 bp	1.0	66.4
ccsA_p3	AGTGGCGGCATATCATTTCT	GCTCTCGCCAATTCACACAA	45.0	50.0	57.4	59.1	1151 bp	1.0	65.9
ccsA_p4	CGAGTGGCGGCATATCATTT	CTCGCCAATTCACACAAAGT	50.0	45.0	58.8	56.9	1150 bp	1.0	65.4
ccsA_p5	CGAGTGGCGGCATATCATTT	TATGATTCGACAGGCTCTCG	50.0	50.0	58.8	56.9	1166 bp	1.0	65.4