Hotspot

atpA

LSC · 9961-11484 · 1524 bp

Back to Stenogyne

Pi 0.000437

MarkerSeek score 59.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	GGTTCAAATCCTATTGGACGC	TGTTGGGAGCGATGAAAGAA	47.6	45.0	57.3	57.4	1761 bp	1.0	62.0
atpA_p2	TCCGAGACACTTGATTCCTT	TGTTGGGAGCGATGAAAGAA	45.0	45.0	56.2	57.4	1689 bp	1.0	61.0
atpA_p3	GGTTCAAATCCTATTGGACGC	ATGTTGGGAGCGATGAAAGA	47.6	45.0	57.3	57.2	1762 bp	1.0	60.2
atpA_p4	TCCGAGACACTTGATTCCTT	ATGTTGGGAGCGATGAAAGA	45.0	45.0	56.2	57.2	1690 bp	1.0	59.2
atpA_p5	TCCGAGACACTTGATTCCTTT	TGTTGGGAGCGATGAAAGAA	42.9	45.0	57.0	57.4	1689 bp	1.0	59.2