Hotspot

psbT-psbN

LSC · 72044-72116 · 73 bp

Back to Stenogyne

Pi 0.0183

MarkerSeek score 69.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 2
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	177-181 bp	1.0	69.2
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	129-133 bp	1.0	69.1
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	185-189 bp	1.0	68.4
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	188-192 bp	1.0	68.3
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	47.6	55.0	57.6	57.5	178-182 bp	1.0	67.1