Hotspot

trnP-UGG-psaJ

LSC · 64804-65073 · 270 bp

Back to Stenogyne

Pi 0.00247

MarkerSeek score 39.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 270
variable_sites: 1
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCCTCGTTACTGCTCAAGGA	CTAAAGCCCCGAACCATAGC	50.0	55.0	58.1	58.4	443 bp	1.0	76.3
trnP-UGG-psaJ_p2	TCCTCGTTACTGCTCAAGGA	GCTAAAGCCCCGAACCATAG	50.0	55.0	58.1	58.4	444 bp	1.0	76.3
trnP-UGG-psaJ_p3	TCCTCGTTACTGCTCAAGGA	GCCGCGGAGAGATATGTTTT	50.0	50.0	58.1	58.4	408 bp	1.0	76.2
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	CTAAAGCCCCGAACCATAGC	45.0	55.0	57.9	58.4	372 bp	1.0	76.1
trnP-UGG-psaJ_p5	AAAACAAACGCGCTACCAAG	GCTAAAGCCCCGAACCATAG	45.0	55.0	57.9	58.4	373 bp	1.0	76.1