Hotspot

rbcL-accD

LSC · 55385-56092 · 708 bp

Back to Stenogyne

Pi 0.00189
MarkerSeek score 45.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
708
variable_sites
2
pi
0.00189
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTGAATGCTACTGCGGGTAC ACTAGAATTGTCACGACCGC 50.0 50.0 58.0 58.0 1613-1614 bp 1.0 80.1
rbcL-accD_p2 TTGAATGCTACTGCGGGTAC GCGCTCGAACTCCTTCTTAA 50.0 50.0 58.0 58.0 1487-1488 bp 1.0 80.1
rbcL-accD_p3 TCATGGTATGCACTTCCGTG ACTAGAATTGTCACGACCGC 50.0 50.0 58.0 58.0 1413-1414 bp 1.0 80.0
rbcL-accD_p4 TCATGGTATGCACTTCCGTG GCGCTCGAACTCCTTCTTAA 50.0 50.0 58.0 58.0 1287-1288 bp 1.0 80.0
rbcL-accD_p5 CAAGATTGGGTCTCTCTGCC GCGCTCGAACTCCTTCTTAA 55.0 50.0 58.0 58.0 1109-1110 bp 1.0 79.9