Hotspot

petN-psbM

LSC · 28804-29305 · 502 bp

Back to Stenogyne

Pi 0.00398
MarkerSeek score 46.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
502
variable_sites
3
pi
0.00398
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCCTACCGCCTTTCTGCTTA 50.0 50.0 58.0 58.4 588-595 bp 1.0 76.9
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCCTACCGCCTTTCTGCTTA 50.0 50.0 58.0 58.4 586-593 bp 1.0 76.9
petN-psbM_p3 CGTAGTGTGGGGAAGAAGTG TCCTACCGCCTTTCTGCTTA 55.0 50.0 57.9 58.4 585-592 bp 1.0 76.3
petN-psbM_p4 ACTCGTAGTGTGGGGAAGAA CCTACCGCCTTTCTGCTTAT 50.0 50.0 58.0 57.4 587-594 bp 1.0 75.4
petN-psbM_p5 TCGTAGTGTGGGGAAGAAGT CCTACCGCCTTTCTGCTTAT 50.0 50.0 58.0 57.4 585-592 bp 1.0 75.4