Hotspot

trnS-GCU-trnG-UCC

LSC · 8179-8872 · 694 bp

Back to Stenogyne

Pi 0.00288
MarkerSeek score 42.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
694
variable_sites
3
pi
0.00288
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGGTTACTAGAACGAATCACA 50.0 40.9 58.0 57.2 775-780 bp 1.0 58.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGGTTACTAGAACGAATCACA 50.0 40.9 58.0 57.2 774-779 bp 1.0 58.1
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ACGGTTACTAGAACGAATCACA 50.0 40.9 58.2 57.2 829-834 bp 1.0 56.9
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA CGGTTACTAGAACGAATCACAC 50.0 45.5 58.0 56.9 774-779 bp 1.0 55.7
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC CGGTTACTAGAACGAATCACAC 50.0 45.5 58.0 56.9 773-778 bp 1.0 55.7