Hotspot

ycf2

Genome · 87440-93709 · 6270 bp

Back to Stenocoelium

Pi 0.00367

MarkerSeek score 48.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6270
variable_sites: 23
pi: 0.00367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCTTTGCTAACACGGATTCC	ATACGGGCCTTTTCCACATC	50.0	50.0	56.8	57.9	199 bp	1.0	71.9
ycf2_p2	CCTTTGCTAACACGGATTCC	GATACGGGCCTTTTCCACAT	50.0	50.0	56.8	57.9	200 bp	1.0	71.9
ycf2_p3	CCTTTGCTAACACGGATTCCT	ATACGGGCCTTTTCCACATC	47.6	50.0	58.3	57.9	199 bp	1.0	71.3
ycf2_p4	CCTTTGCTAACACGGATTCCT	GATACGGGCCTTTTCCACAT	47.6	50.0	58.3	57.9	200 bp	1.0	71.3
ycf2_p5	TCCTTTGCTAACACGGATTCC	ATACGGGCCTTTTCCACATC	47.6	50.0	58.3	57.9	200 bp	1.0	71.2