Hotspot

ndhI

Genome · 120714-121217 · 504 bp

Back to Stenocoelium

Pi 0.00794

MarkerSeek score 61.30

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 504
variable_sites: 4
pi: 0.00794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CCAAGCAAGAGCCTCATTCT	TCTTCACGACTTGTCTTAAACA	50.0	36.4	57.9	55.7	644 bp	1.0	44.8
ndhI_p2	ACCAAGCAAGAGCCTCATTC	TCTTCACGACTTGTCTTAAACA	50.0	36.4	58.2	55.7	645 bp	1.0	44.4
ndhI_p3	ATTCACCAAGCAAGAGCCTC	TCTTCACGACTTGTCTTAAACA	50.0	36.4	58.2	55.7	649 bp	1.0	44.4
ndhI_p4	CCAAGCAAGAGCCTCATTCT	TCTTCACGACTTGTCTTAAACAA	50.0	34.8	57.9	56.4	644 bp	1.0	42.5
ndhI_p5	ACCAAGCAAGAGCCTCATTC	TCTTCACGACTTGTCTTAAACAA	50.0	34.8	58.2	56.4	645 bp	1.0	42.1