Hotspot

ndhB-trnL-CAA

Genome · 145502-146074 · 573 bp

Back to Stenocoelium

Pi 0.00873

MarkerSeek score 63.20

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: Genome
length_bp: 573
variable_sites: 5
pi: 0.00873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	633 bp	1.0	80.1
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	631 bp	1.0	80.1
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	621 bp	1.0	79.5
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	628 bp	1.0	78.6
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	632 bp	1.0	78.0