Hotspot

ndhF-rpl32

Genome · 113535-114592 · 1058 bp

Back to Stenocoelium

Pi 0.00865

MarkerSeek score 65.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1058
variable_sites: 9
pi: 0.00865
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1238-1243 bp	1.0	80.1
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1236-1241 bp	1.0	80.0
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1237-1242 bp	1.0	80.0
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1249-1254 bp	1.0	78.9
ndhF-rpl32_p5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	50.0	50.0	57.8	58.2	1226-1231 bp	1.0	78.9