Hotspot

rbcL-accD

Genome · 58340-58952 · 613 bp

Back to Stenocoelium

Pi 0.00165

MarkerSeek score 61.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: Genome
length_bp: 613
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTGCTTGTGAAGTATG	CACACCTGTGTTCGAACTCT	50.0	50.0	57.5	57.8	731-734 bp	1.0	74.9
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	TAGACCACACCTGTGTTCGA	50.0	50.0	57.5	58.3	736-739 bp	1.0	74.2
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	TTCAATAGGACCAAGACCGC	50.0	50.0	57.5	57.6	769-772 bp	1.0	73.4
rbcL-accD_p4	ACTAAATGGAGCCCCGAACT	CACACCTGTGTTCGAACTCT	50.0	50.0	58.7	57.8	752-755 bp	1.0	73.4
rbcL-accD_p5	ACTAAATGGAGCCCCGAACT	TAGACCACACCTGTGTTCGA	50.0	50.0	58.7	58.3	757-760 bp	1.0	72.8