Hotspot

ndhC-trnV-UAC

Genome · 52043-53113 · 1071 bp

Back to Stenocoelium

Pi 0.0019
MarkerSeek score 61.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
1071
variable_sites
2
pi
0.0019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCAGGCCCTTTGTTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1207-1223 bp 1.0 79.5
ndhC-trnV-UAC_p2 TAATCGGGGCTAAAACTCCG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 1194-1210 bp 1.0 75.6
ndhC-trnV-UAC_p3 TCAGGCCCTTTGTTAATCGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1192-1208 bp 1.0 74.9
ndhC-trnV-UAC_p4 CCCTTTGTTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1202-1218 bp 1.0 74.7
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.8 58.1 1189-1205 bp 1.0 73.8