Hotspot

trnE-UUC-trnT-GGU

Genome · 31948-32740 · 793 bp

Back to Stenocoelium

Pi 0.00526
MarkerSeek score 65.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
Genome
length_bp
793
variable_sites
4
pi
0.00526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 870-897 bp 1.0 81.9
trnE-UUC-trnT-GGU_p2 GTGTAGTACCCTACCCCCAG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 905-932 bp 1.0 81.5
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 835-862 bp 1.0 81.3
trnE-UUC-trnT-GGU_p4 GTGTAGTACCCTACCCCCAG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.2 57.9 870-897 bp 1.0 80.8
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 867-894 bp 1.0 80.8