Hotspot

rpoB-trnC-GCA

Genome · 27656-28896 · 1241 bp

Back to Stenocoelium

Pi 0.00337

MarkerSeek score 64.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1241
variable_sites: 4
pi: 0.00337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1334-1385 bp	1.0	81.8
rpoB-trnC-GCA_p2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1335-1386 bp	1.0	81.8
rpoB-trnC-GCA_p3	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1316-1367 bp	1.0	79.7
rpoB-trnC-GCA_p4	CCACGAACCTACAAAAGCCT	TATTGAATCAGGCGACACCC	50.0	50.0	58.1	57.7	1403 bp	0.5	56.5
rpoB-trnC-GCA_p5	TCCACGAACCTACAAAAGCC	TATTGAATCAGGCGACACCC	50.0	50.0	58.1	57.7	1404 bp	0.5	56.5