Hotspot

rps16

LSC · 4602-5720 · 1119 bp

Back to Stemona

Pi 0.0135

MarkerSeek score 56.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1119
variable_sites: 30
pi: 0.0135
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGCCCGAGGTGAAAGAAAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	2065-2095 bp	1.0	83.2
rps16_p2	TTGCCCGAGGTGAAAGAAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	2066-2096 bp	1.0	83.2
rps16_p3	TTTGCCCGAGGTGAAAGAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	2067-2097 bp	1.0	83.2
rps16_p4	AAGGGCAAATTGGGGGAAAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1741-1770 bp	0.8	75.2
rps16_p5	CCTTTCAGGATCAGTCGTGG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	2319-2349 bp	0.8	74.6