Hotspot

trnL-UAG-rpl32

SSC · 123157-123940 · 784 bp

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Pi 0.0154

MarkerSeek score 70.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 28
pi: 0.0154
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	CGATTTCTACGGGGCATTCA	50.0	50.0	57.9	58.1	873-881 bp	1.0	83.0
trnL-UAG-rpl32_p2	AGCGTGTCTACCGATTTCAC	CGATTTCTACGGGGCATTCA	50.0	50.0	58.0	58.1	864-871 bp	0.8	75.4
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	CGATTTCTACGGGGCATTCA	50.0	50.0	58.0	58.1	865-872 bp	0.8	75.4
trnL-UAG-rpl32_p4	AGCGTGTCTACCGATTTCAC	TCGATTTCTACGGGGCATTC	50.0	50.0	58.0	57.8	865-872 bp	0.8	73.9
trnL-UAG-rpl32_p5	CAGCGTGTCTACCGATTTCA	TCGATTTCTACGGGGCATTC	50.0	50.0	58.0	57.8	866-873 bp	0.8	73.9