Hotspot

ycf1-rps15

SSC · 113583-113887 · 305 bp

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Pi 0.0273

MarkerSeek score 60.20

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 305
variable_sites: 16
pi: 0.0273
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGGCCCAACTACCGATTAGA	CAGCAGTCGTTGACGTTTTC	50.0	50.0	58.1	58.0	617-645 bp	1.0	82.6
ycf1-rps15_p2	GGGAGTCGTTTCAGTCTGTC	CAGCAGTCGTTGACGTTTTC	55.0	50.0	57.9	58.0	554-582 bp	0.8	75.2
ycf1-rps15_p3	GGGAGTCGTTTCAGTCTGTC	TGCATTGATGGGTGATGAGG	55.0	50.0	57.9	57.9	797-825 bp	0.8	74.0
ycf1-rps15_p4	TGGCCCAACTACCGATTAGA	TTGAACATTTCCCAGCTGCT	50.0	45.0	58.1	58.0	1071-1099 bp	0.8	73.0
ycf1-rps15_p5	GGGAGTCGTTTCAGTCTGTC	TTGAACATTTCCCAGCTGCT	55.0	45.0	57.9	58.0	1008-1036 bp	0.6	65.3