Hotspot

psaJ-rpl33

LSC · 65794-66293 · 500 bp

Back to Stemona

Pi 0.0105

MarkerSeek score 51.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 11
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	TTTCTGGTTCTAGGACCGGT	50.0	50.0	58.0	58.0	1837-1866 bp	1.0	82.4
psaJ-rpl33_p2	GCGTTCTACCGAACTGAACT	TTTCTGGTTCTAGGACCGGT	50.0	50.0	57.9	58.0	1846-1875 bp	1.0	82.0
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	GGGTGTATTGTGGCGATTCT	50.0	50.0	58.0	58.0	1291-1322 bp	1.0	81.9
psaJ-rpl33_p4	CGAACTGAACTAAGAGCGCT	CCCCGGCGATTCTTTATTGA	50.0	50.0	58.0	58.0	1249-1259 bp	0.8	73.6
psaJ-rpl33_p5	CGAACTGAACTAAGAGCGCT	TCCCCGGCGATTCTTTATTG	50.0	50.0	58.0	58.0	1250-1260 bp	0.8	73.6