Hotspot

psbC-trnS-UGA

LSC · 34012-34139 · 128 bp

Back to Stemona

Pi 0.0328
MarkerSeek score 60.00
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
128
variable_sites
7
pi
0.0328
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 AACCCGTTCTTTCCATGACC ATAGCCCCGGTCTTGAAAAC 50.0 50.0 58.1 57.9 232-246 bp 1.0 80.9
psbC-trnS-UGA_p2 TGGAACCCGTTCTTTCCATG ATAGCCCCGGTCTTGAAAAC 50.0 50.0 58.1 57.9 235-249 bp 1.0 80.9
psbC-trnS-UGA_p3 GGAACCCGTTCTTTCCATGA ATAGCCCCGGTCTTGAAAAC 50.0 50.0 57.8 57.9 234-248 bp 1.0 80.1
psbC-trnS-UGA_p4 ATCGTGATTTGGAACCCGTT ATAGCCCCGGTCTTGAAAAC 45.0 50.0 57.8 57.9 244-258 bp 1.0 80.0
psbC-trnS-UGA_p5 CGTTCTTTCCATGACCCCTC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.3 57.9 228-242 bp 1.0 79.6