Hotspot

psbM-trnD-GUC

LSC · 28330-29296 · 967 bp

Back to Stemona

Pi 0.0056

MarkerSeek score 60.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 12
pi: 0.0056
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAAATGAACAGTGCCGTAGC	AGCCTCACTGGGATTGTAGT	45.0	50.0	57.0	58.0	1094-1107 bp	1.0	73.6
psbM-trnD-GUC_p2	AAAATGAACAGTGCCGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.0	58.5	1075-1088 bp	1.0	70.2
psbM-trnD-GUC_p3	AAAATGAACAGTGCCGTAGC	GCCTCACTGGGATTGTAGTT	45.0	50.0	57.0	57.2	1093-1106 bp	1.0	67.7
psbM-trnD-GUC_p4	AAAATGAACAGTGCCGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.0	57.0	1076-1089 bp	1.0	66.0
psbM-trnD-GUC_p5	AAAATGAACAGTGCCGTAGC	CAGCCTCACTGGGATTGTAG	45.0	55.0	57.0	57.7	1095-1098 bp	0.6	54.2