Hotspot

petN-psbM

LSC · 27301-28224 · 924 bp

Back to Stemona

Pi 0.0111

MarkerSeek score 64.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 22
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACGGCACTGTTCATTT	55.0	45.0	58.1	58.1	1028-1248 bp	1.0	79.9
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACGGCACTGTTCATTT	55.0	45.0	58.1	58.1	1027-1247 bp	1.0	79.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACGGCACTGTTCATTT	50.0	45.0	58.2	58.1	1086-1306 bp	1.0	79.6
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACGGCACTGTTCATTT	55.0	45.0	57.8	58.1	1026-1246 bp	1.0	79.5
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	TGCTACGGCACTGTTCATTT	60.0	45.0	58.2	58.1	1023-1243 bp	1.0	79.1