Hotspot

trnC-GCA-petN

LSC · 26752-27210 · 459 bp

Back to Stemona

Pi 0.0249

MarkerSeek score 61.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 21
pi: 0.0249
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	553-652 bp	1.0	81.7
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	552-651 bp	1.0	81.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	494-593 bp	1.0	81.7
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	554-653 bp	1.0	81.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	CTAGAGTCCACTCCTTCCCC	50.0	60.0	57.5	58.2	557-656 bp	1.0	80.9