Hotspot

trnS-GCU-trnG-UCC

LSC · 7472-7986 · 515 bp

Back to Stemona

Pi 0.0116
MarkerSeek score 65.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
515
variable_sites
13
pi
0.0116
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCACT 45.0 42.9 58.0 58.2 572-609 bp 1.0 74.8
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCAC 45.0 45.0 58.0 56.8 572-609 bp 1.0 74.8
trnS-GCU-trnG-UCC_p3 TCGAACCCTCGGTACCAATA ACGAATCGCACTTTTACCACT 50.0 42.9 57.9 58.2 605-642 bp 1.0 73.7
trnS-GCU-trnG-UCC_p4 TCGAACCCTCGGTACCAATA ACGAATCGCACTTTTACCAC 50.0 45.0 57.9 56.8 605-642 bp 1.0 73.7
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA ACGAATCGCACTTTTACCACT 55.0 42.9 58.3 58.2 620-657 bp 1.0 72.2