Hotspot

psbI-trnS-GCU

LSC · 7239-7383 · 145 bp

Back to Stemona

Pi 0.0124

MarkerSeek score 41.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 4
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	237-245 bp	1.0	73.6
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	261-269 bp	1.0	72.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.1	58.2	192-200 bp	1.0	72.4
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.1	58.3	189-197 bp	1.0	71.6
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.8	58.0	237-245 bp	1.0	71.2